Invitrogen Qubit RNA IQ Assay Kit

Invitrogen Qubit RNA IQ Assay Kit

Sample Volume: 1-20 µL (0.5-1.5 µg)

For Use With (Equipment): Qubit™ 4 Fluorometer

Product Line: Qubit™

Product Size: 75 assays

Sample Type (General): RNA

Shipping Condition: Dry Ice

內(nèi)容及儲存

Store kit at -20°C or below. Store RNA standards at -80°C or below.

Invitrogen江蘇銷售Qubit RNA IQ Assay Kit

Invitrogen江蘇銷售Qubit RNA IQ Assay Kit

Qubit熒光計(jì)工作原理
單個樣品檢測非常簡單
便于使用的熒光計(jì)，可快速簡單地獲得實(shí)驗(yàn)結(jié)果 大尺寸的彩色觸摸屏，可輕松進(jìn)行各種不同操作。 計(jì)算和設(shè)置都由儀器自動完成 (圖 1)。 Qubit 4熒光計(jì)可保存高達(dá)1000個樣品的數(shù)據(jù)，數(shù)據(jù)可由USB數(shù)據(jù)線或U盤傳輸。 此外，新的Qubit 4還包含一個內(nèi)置的試劑計(jì)算器，可以輕松確定準(zhǔn)備樣品和標(biāo)準(zhǔn)所需的染料和緩沖液的量 （圖2）。

適用于Qubit熒光計(jì)的試劑盒
用于Qubit熒光計(jì)的Qubit試劑盒，配合簡單的混合讀取模式，均采用相同的操作方式。其中DNA和RNA分析試劑盒僅需孵育2分鐘，而蛋白分析試劑盒需要15分鐘 (圖 3)。 Qubit 4熒光計(jì)自動保存您的數(shù)據(jù)，您可用USB數(shù)據(jù)線或U盤導(dǎo)出。 除了常規(guī)的 dsDNA，寡核苷酸，總RNA，小RNA和蛋白質(zhì)的分析試劑盒，在 Ion Personal Genome Machine(PGM) 測序儀上進(jìn)行測序之前，可使用專門的Ion Sphere Quality Control Kit在Qubit 4熒光計(jì)上進(jìn)行Ion Sphere顆粒質(zhì)量評估。

The Qubit RNA IQ Assay Kit provides a fast, simple method to check whether an RNA sample has degraded using the Qubit 4 Fluorometer (required). The assay utilizes two unique dyes: one that binds to large, intact and/or highly structured RNA (mRNA, tRNA, rRNA), and another that selectively binds to small and/or, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample.

To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer. Results are presented as an RNA IQ number (RNA IQ#) that indicates the RNA sample integrity and quality, and also as a calculated percent of large and small RNA in the sample. The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

Solution-based compared to electrophoresis methods
The RNA IQ assay results correlate well with electrophoretic methods. The primary difference between the solution-based RNA IQ assay and traditional electrophoresis-based techniques is the amount of time it takes to ascertain whether an RNA sample has degraded. Gels are considered easy to perform, but still take time to run. Microfluidic-based methods not only take time to obtain results, but often have lengthy procedures to prepare the samples. Gel or microfluidic-based electrophoresis is still recommended to obtain fragment size information or size distribution of the RNA sample.

Notes:
1. The Qubit RNA IQ assay will only work on the Qubit 4 Fluorometer. It will not work on the Qubit, Qubit 2.0, or Qubit 3 fluorometers.
2. 500 µL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.